1. Crystal violet, 2. Gram's iodine, 3. Alcohol, and 4. Safranin is listed in the proper order to represent the components used in the Gram staining technique.
Applying a base color (crystal violet). adding Gram's iodine as a mordant. Rapid decolorization using acetone, ethanol, or a combination of the two. using safranin as a counterstain.
The outcome of the Gram stain will depend on how thick the smear is utilized. The decolorizing process is the one that has the biggest impact on how the stain will turn out.
There are three steps in the procedure: Crystal violet staining dye is applied to cells. Then, to create a complex between the iodine and the crystal violet, a Gram's iodine solution of iodine & potassium iodide is added.
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