As the ratio of holo-RBP to STRA6 increases, less retinol is released.
Fluorescence is binding, not release, which is why there is more fluorescence. As the molar ratio of holo-RBP to STRA6 decreases, retinol release increases. You can see that 0.5uM holo-RBP had more retinol release, so less relative retinol fluorescence, than 4uM holo-RBP, so a lower molar ratio of holo-RBP is related to an increase in retinol release. Retinol fluorescence was similar under STRA6 alone and STRA6/LRAT conditions when the holo-RBP to STRA6 molar ratio was low (0.5 μM holo-RBP). When the membranes were incubated with 4 μM holo-RBP, the fluorescence of retinol in the STRA6 condition alone was higher than that in the STRA6/LRAT condition, indicating that as the molar ratio of holo-RBP to STRA6 increases, STRA6 becomes more dependent on LRAT for retinol release.
To learn more about retinol click here https://brainly.com/question/8540401
#SPJ4
