Answer: Overheating an enzyme results in the enzyme's loss of ... Net electrical charge. ... Enzymes are proteins. Increases in temperature increase molecular activity, and can result in a higher rate of collisions between enzymes and substrates. If the temperature rises too high, however, the enzymes could become denatured, and the positive effects of the temperature increase could be nullified. What happens to an enzyme if the temperature get too high? The reaction stops, because the rise in temperatures makes the enzyme's molecules vibrate more. If the temperature goes above a certain level, this vibration breaks some of the bonds that hold the enzyme in shape.
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