What is the reason for analyzing a blank in the spectrophotometer before analyzing the standard solution? A. any absorbance of light by the solvent AND the cuvette needs to be accounted for B. any absorbance of light by the cuvetto needs to be accounted for C. so the scattered light is filtered before reaching the photodiode D. to account for any fingerprints on the cuvette E. any absorbance of light by the cuvette and fingerprints needs to be accounted for

Blank calibrations are usually carried out on the spectrometer to eliminate and account for any readings that would come from absorptions of the curvette, solvents/reagents or anything that is not the main analyte we are testing for.

Hence the blank solution does not contain the analyte itself. Fingerprints should be removed from the curvette as practice. Hence the most suitable answer amongst the options is A.

I hope this was clear ad most of all, helpful.

tutorareej tutorareej · Answer 2 · 2021-12-07T14:16:10+01:00

The blank has been resulted in for accounting the absorbance of light by the solvent and the cuvette. Thus, option A is correct.

For performing the spectroscopic measurement of an analyte, the system has been blanked with the cuvette and the solvent. This has been performed as the solvent medium, the environment and the cuvette have their own absorption.

The analysis of the analyte without performing blank has consisted of the absorbance of the solvent and cuvette as well. Thus, in order to attain a pure analyte absorbance, the spectrometer has been analyzed with the blank.

The blank has been resulted in for accounting the absorbance of light by the solvent and the cuvette. Thus, option A is correct.

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