Question

A single base-pair mutation occurs in a promoter sequence of a gene. As a result of the mutation, what are the possible effects on the transcription level of the gene?

Answer 1

Answer:

This mutation could increase, reduce, have no effect, or even eliminate the transcriptional activity

Explanation:

A single pair mutation (also known as point mutation) is caused when only one nucleotide in the DNA sequence is changed. A promoter is a DNA sequence that binds to the RNA polymerase and transcription factors in order to initiate the transcription. When a point mutation results in a promoter region, this may alter transcription, and its effects can be determined by measuring the level of gene transcription. In a promoter bashing assay, point mutations are generated in the promoter region in order to determine their effects on gene expression.