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Each of the following statements describes a type of column chromatography. Sort the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the "All" category. (Note that size-exclusion chromatography may also be called gel-filtration or molecular exclusion chromatography.)

Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and All

Statements:
Separate molecules by size.
Separate molecules by charge.
The stationary phase has a covalently bound group to which a protein in the mobile phase can bind.
Uses a mobile phase and a stationary phase to separate proteins.
The stationary phase contains cross-linked polymers with different pore sizes.
Can separate molecules based on protein-lignand binding.
The stationary phase may contain negatively or positively charged groups.

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Answer:

Explanation:

  • Separate molecules by size. > All
  • Separate molecules by charge. > Ion-exchange Chromatography, Affinity Chromatography
  • The stationary phase has a covalently bound group to which a protein in the mobile phase can bind. > All
  • Uses a mobile phase and a stationary phase to separate proteins.  > All
  • The stationary phase contains cross-linked polymers with different pore sizes. > Size-exclusion Chromatography
  • Can separate molecules based on protein-ligand binding.  > Affinity Chromatography
  • The stationary phase may contain negatively or positively charged groups. > Ion-exchange Chromatography, Affinity Chromatography

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