Answer:
1. The purpose of the uninoculated control tubes used in the oxidation fermentation test is to serve as basis of the medium for the color comparison and that it is sterile.
2. It is necessary to use two controls in order to illustrate the no color change (green color) under both aerobic and anaerobic conditions.
3. The purpose of the experiment is to determine if the organisms utilize the carbohydrate and thus is fermentative. This will result in a color change from green to yellow.
Thus, the medium in one tube is sealed with a layer of paraffin to prevent diffusion of oxygen. This tube will show that the medium is unaffected by the lack of oxygen.
The second tube will allow the diffusion of oxygen. This tube will show that the medium does not change color naturally in the presence of oxygen. Thus, the color changes will be caused only by the fermentation process of the organisms.
The purpose of uninoculated control tube used in oxidation fermentation test served as medium for color comparison during the fermentation test because the tube does not have color change under two various conditions.
Uninoculated control tube refer to a microbiological tube that is use to isolate living organisms and for negative control.
Two uninoculated control tubes are necessary so as to show the results of the medium in both aerobic and anaerobic environments. It reveal that the medium still contain green under the to conditions.
The second tube is use to allow oxygen diffusion, which indicate that color does not change naturally.
The carbohydrate fermentation test is a type of test use to know whether a bacteria can use a form of carbohydrate or not.. It test the presence of acid and gas produced from the fermentation of a particular carbohydrate.
Therefore, The purpose of uninoculated control Tubes used in fermentation test serve as medium for color comparison during the fermentation test because the tube does not have color change under two various conditions.
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