Spore test is performed to ensure the effectiveness of autoclave because they are highly resistant cell that protects the bacterial genetic material.
Further Explanation:
Sterilization is the process used to destruct all form of microorganism such as bacteria, fungi and endospore. Autoclaving and autoclave sterilization is the common method in which high temperature is used to destroy microbes and spores. This technique is able to destroy those microbes which are hard to destroy by conventional method.
Endospore are the outer covering of the gram positive bacteria which provide the high resistant to cell to protect its genetic material. They are able to survive high environmental assaults such as high temperature, chemical destruction, U.V radiation and desiccation that will kill normal microbes.
Spore test is the method used to measure the effectiveness of the autoclave. In this test non pathogenic spores are used. These spores are placed along with other equipment in the autoclave and run it as per the given instruction. After autoclaving analyze it by culture method. In case of positive result the autoclave is not functioning properly.
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Answer Details:
Grade: College Biology
Subjects: Health
Topic: Sterilization
Keywords:
Spores, sterilization, destruct, microorganism, bacteria, endospore, resistant, genetic, assault, autoclaving, chemical, desiccation, pathogenic, culture.
