At the inner mitochondrial membrane, a high energy electron is passed along an
electron transport chain. The energy that is being released pumps hydrogen out
of the matrix space. The gradient is
created by this process, drives hydrogen back through the membrane, through the
ATP synthase. Thus, the ATP is very essential for life, and that a
proton gradient and membrane prospective are vital in order for ATP synthesis
to occur in the mitochondria. However, mitochondria is very active that it can converse
its process. Complex V is the enzyme liable for final ATP synthesis at the end
of oxidative phosphorylation.