While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?
Amylase converts starch to glucose. So the one that didn't have starch in it would not show glucose formation because the amylase didn't have starch to convert to glucose.
