During translation, the correct amino acid is added to the growing polypeptide chain by aminoacyl-tRNA (transfer RNA).
Aminoacyl-tRNAs deliver the correct amino acids to the ribosome and are added to polypeptide chains based on the sequence of codons (triplets of nucleotide bases) in the mRNA (messenger RNA).
Each aminoacyl tRNA molecule has a specific anticodon, the complementary sequence of three nucleotide bases complementary to the codons in the mRNA, so that the correct amino acid is added to the polypeptide chain.
The anticodon of the aminoacyl-tRNA can bind to the codon of the mRNA through complementary base pairing. This allows the ribosome to precisely match the codons of the mRNA with the corresponding amino acids carried by the aminoacyl-tRNA.
The other factors such as (number of twists in properly supercoiled DNA, poly-A tails in properly modified mRNAs, and methylguanosine caps in properly modified mRNAs) determine the correct amino acid sequence during translation are not directly guaranteed to be added. However, by helping to stabilize the structure and function of the mRNA molecule, they can play a role in the overall fidelity of the translation process.
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