Question

In an experiment, a scientist isolates mitochondria from living cells and suspends them in two different buffered solutions. One solution is maintained at pH 4, while the other solution is maintained at pH 9. The scientist finds that mitochondria in the solution at pH 4 continue to produce ATP but those in the pH 9 solution do not.The results of the experiment can be used as evidence in support of which of the following scientific claims about mitochondrial activity?

Answer 1

The results of the experiment can be used as evidence in support of the  scientific claims that ATP production in mitochondria requires a hydrogen ion gradient that favors movement of protons into the mitochondrial matrix.

Glucose metabolism is completed in the mitochondria, and the energy emitted is utilized so efficiently that approximately 30 ATP molecules are generated for every glucose molecule that is oxidized. By contrast, glycolysis produces only two molecules of ATP per glucose molecule.

The majority of the adenosine triphosphate (ATP) synthesized throughout glucose metabolism is generated in the mitochondria through oxidative phosphorylation. This is a complex reaction that is controlled by the proton gradient obtained by mitochondrial respiration across the inner membrane of the mitochondria.

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