Question

In insertional inactivation
A) if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies remain white
B) if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colonies turn blue
C) if foreign DNA is inserted, the beta galactosidase marker is activated, and the colonies remain white
D) if foreign DNA is inserted, the beta galactosidase marker is activated, and the colonies turn blue

Answer 1

In insertional inactivation A) if foreign DNA is inserted, the beta galactosidase marker is inactivated, and the colony remains white

Inactivation is a recombinant DNA technique used to select bacteria carrying a recombinant plasmid by inserting a DNA fragment into the restriction gene for antibiotic resistance.

Insertional inactivation is a technique used in recoombinant DNA with the insertion of solid target vector genes. This technique will cause the gene to become nonfunctional. The recombinant DNA is inserted into the coding sequence of β-galactosidase. Inactivation of β-galactosidase and colonies will not produce any color. Colonies that remain white are known as recombinant colonies.

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