Assume that a plate of Clostridium Sporogenes is being incubated in a CO₂ incubator, so this lack of growth is caused by the absence of oxygen, and this organism is an obligate aerobe.
When you look at the plate, if there is another microorganism growing then there’s contamination while you inoculate. If there is no microorganism at all then Clostridium Sporogenes need oxygen as one of its growth factors.
Obligate aerobe is a need for oxygen to grow through cellular respiration. Cellular respiration is a process to metabolize fats and sugars to produce energy or ATP Adenosine Triphosphate. Aerobic respiration gains more energy than fermentation.
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