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Because they are totipotent, embryonic stem cells have a special quality that makes them beneficial for creating knockout animals. A single totipotent cell is one that, with the right amount of maternal assistance, may develop into a new creature (most stringent definition).

A totipotent cell is one that has the ability to develop into every extraembryonic tissue, as well as every bodily tissue and the germline (less stringent definition). Totipotent stem cells have the capacity to divide into any form of cell in an organism. Till a full, finished creature is produced, a totipotent cell can divide.

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