Question

1. Using the CRISPR/Cas9 system, a genetically-engineered sgRNA complementary to a target site in the genome binds to Cas9 endonuclease. A mutation can occur at this site when A. Cas9 cleaves the DNA and nonhomologous end-joining results in a small deletion.
B. Cas9 cleaves the DNA and homologous recombination with the homologous chromosome is used to repair the break.
C. Cas9 cleaves the DNA and nonhomologous end-joining correctly repairs the break.
D. All of the above
Please explain in great detail. I believe the correct answer is D but not 100 percent.

Answer 1

Option A, A: The "spacer" sequences of CRISPR are translated into short RNAs called "CRISPR RNAs" or "crRNAs," which can direct the system to DNA sequences that match them. When the targeted DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops it, turning off the gene.

In a normal CRISPR investigation, a sgRNA is created with a guide sequence domain at the 5′ end that is complementary to the DNA target sequence (identified as gRNA in our work). The CRISPR Cas9 protein is then directed to particular DNA locations in the genome for targeted cleavage using the rationally designed sgRNA.

learn more about CRISPR here:

https://brainly.com/question/29509174

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