Promoters direct RNA polymerase to the initiation site.
RNA synthesis complexes containing DNA, RNA, and polymerase are called transcription bubbles.
The expression of beta-galactosidase is enhanced by the addition of repressors.
A promoter is the DNA sequence, where RNA polymerase starts to transcribe a gene. Promoter sequences are located directly upstream or it can be at the 5′ end of the transcription initiation site. Molecular structure formed during DNA transcription is known as transcription bubbles, when a very small portion of the DNA double helix is unwound. The size range of a transcription bubble is from 12-14 base pairs. Repressors are proteins that reduces or turn of gene expression, which is reflected by reduced mRNA production from the affected gene.
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