Malachite green is used to stain the endospore, and safranin is the stain used to stain the vegetative cell in schaeffer-fulton method of spore staining.
Malachite Green is used for staining bacterial spore by Schaeffer and Fulton's method. It is forced into the spores by steaming the bacterial emulsion. It can be used as a simple stain also, for bacterial cells and in place of methyl-green in Pappenheim stain, when it is combined with Gram stain. Safranin is a basic dye, positively charged which is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. Vegetative cells will be pink, and endospores will be dark green after staining with this.
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