Hybridization is the process by which the cDNA binds to complementary base pairs in each of the locations on the array. Each spot will appear red, green, or yellow when scanned with a laser depending on how the DNA links together.
Since RNases are constantly present, cDNA is a more practical technique to work with the coding sequence than mRNA. This is the main justification for sequencing cDNA rather than mRNA. Similar to this, to create their probes, researchers using DNA microarrays frequently transform the mRNA into cDNA. The most effective technique for examining gene expression in various organisms is the cDNA microarray. It has been effectively used for both extensive gene discovery and the simultaneous expression of tens of thousands of genes.
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