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Due to variations in the cell wall, prokaryotes can either stain gram-positive or gram-negative.

Why are prokaryotes gram-positive or gram-negative stained?

The thick, peptidoglycan coating of gram-positive bacteria's cell walls will hold onto the dye, causing them to stain violet. The bacteria will stain red if they are gram-negative and the dye leaks through the thin peptidoglycan coating.

What distinguishes gram-positive bacteria from gram-negative bacteria?

The hue of a Gram stain is purple. The bacteria in a sample will either stay purple or turn pink or red when the stain and bacteria combine.

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