in correct order, from external to internal, the tissues of the testes include: cremaster muscle tunica vaginalis tunica albuginea septa

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The correct order is dartos, tunica vaginalis, tunica albuginea, septa, scrotal cavity, cremaster


small pedunculated bodies may undergo torsion.
The testis lies outside the body cavity in the scrotum where it is maintained at a temperature 2–3°C lower than the body temperature. Failure to descend into the scrotum causes failure of spermatogenesis.

Blood supply
The testis and epididymis are suspended by the spermatic cord containing their arterial blood supply, venous and lymphatic drainage and nerve supply. The testis develops in the L2/L3 vertebral region and drags its vascular, lymphatic and nerve supply from this region to the scrotum.
Testicular and renal pain may mimic each other. Testicular pain hence can radiate to the loin and renal pain often is referred to the scrotum.

The arterial supply is via the testicular artery which is a branch of the abdominal aorta given off just below the level of origin of the renal arteries. The venous drainage is via the pampiniform plexus of veins which become a single testicular vein before terminating in the inferior vena cava on the right side and the left renal vein on the left side.
The lymphatics of the testis and epididymis accompany the blood vessels and drain into the para-aortic lymph nodes. Upper abdomen must therefore be palpated when searching for secondary lymphatic spread from a carcinoma of the testis.

The spermatic cord
The spermatic cord contains the ductus deferens (vas deferens), the testicular artery and the pampiniform plexus of veins. Other structures in the cord are the cremasteric artery, the artery to the vas, nerve to the cremaster, sympathetic nerves and the lymphatics of the testis and epididymis. Because of its passage through the inguinal canal the spermatic cord acquires three coverings, i.e. the external spermatic fascia from the external oblique aponeurosis at the superficial inguinal ring, the cremasteric muscle and fascia from the internal oblique, and the internal spermatic fascia from the transversalis fascia at the deep inguinal ring.

Varicocoele is a condition caused by dilated tortuous pampiniform plexus of veins. The condition, which may be felt as a ‘bag of worms’ while palpating the scrotum, is more commonly present on the left side. As the left testicular veins drain into the left renal veins it may be a sign of a malignant tumour in the left kidney.

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