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Yes it is true that examining the electrophoresis gel and reading from bottom to top, one base at a time, gives the sequence of DNA.

One needs to look at the dark bands in each column to read the gel. Every nucleotide type has its own column (G, C, A, T). The nucleotide sequence can be ascertained by reading the bands' order. Following the detection of the gel's bands, the DNA sequence is read from the gel's bottom to its top, taking into account the bands in each of the gel's four lanes.

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