Question

A positive food test produces an orange precipitate. Which test was performed? A Amino acids were mixed with cold biuret solution. B Glucose was heated with benedict's solution C Protein was heated with benedict's solution D Protein was mixed with biuret solution and heated

Answer 1

Glucose was heated with benedict's solution which is the test performed for a positive food test producing an orange precipitate.

So, the correct option is B.

When checking for one specific reducing sugar glucose using Benedict's reagent, it first appears turquoise blue. When heated with reducing sugars, the color changes from yellow to orange. The final color of the reagent will appear "hotter" if the concentration of reducing sugar is greater. The solution becomes orange or brick red when Benedict's solution and simple carbohydrates are heated. The ability of simple carbs to reduce causes this response.

Benedict's solution changes hue because the copper (II) ions are converted to copper (I) ions. A decreasing sugar is a glucose. While residues of the acyclic form are also present, the equilibrium of glucose in an aqueous solution considerably favors the glucopyranose form.

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