Respuesta :
The restriction endonuclease HindIII cut linear dna at three cut sites, which resulted in five restriction fragments.
The restriction endonuclease HindIII cut linear DNA at three cut sites, which resulted in five restriction fragments. The sizes of the fragments were: 2.4 kb, 1.8 kb, 1.2 kb, 0.6 kb, and 0.3 kb. The 2.4 kb fragment contained the HindIII site, the 1.8 kb fragment contained the EcoRI site, and the 1.2 kb fragment contained the BamHI site. The 0.6 kb and 0.3 kb fragments did not contain any known restriction sites.
The first cut site was at the base of the DNA molecule, and the second and third cut sites were located at the ends of the DNA molecule. The four restriction fragments were of different lengths, and the fifth fragment was of the same length as the first fragment. The first fragment was the longest, and the second and third fragments were shorter. The fourth fragment was the shortest, and the fifth fragment was the same length as the first fragment.
The HindIII enzyme is a useful tool for researchers who want to study the function of specific DNA sequences. For example, if a researcher wants to study a particular gene, they can use the enzyme to cut the DNA molecule at the site of the gene. This will result in a fragment that contains the gene of interest. The researcher can then study the function of the gene by examining the DNA sequence of the fragment.
A restriction enzyme is an enzyme that recognizes a specific sequence of nucleotides in DNA and cuts the DNA at that point.Restriction fragments can also be used to map the location of genes on a chromosome. If the location of a restriction site is known, then the location of the gene can be determined. In summary, restriction fragments can be used to identify genes, to map their location on a chromosome, and to clone them.
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