Question

If the laci gene in the genetically engineered fluorescent strain were nonmutant but the laco sequence on the plasmid mutated to lacoc, how would the cells fluoresce in the presence of inducer? in the absence of inducer?.

Answer 1

The cell in the  presence of an inducer would fluoresce in yellow and in absence of inducer it would not fluoresce.

When an inducer was present, the cell would glow yellow. Fluorescence doesn't exist without an inducer. The galaxy-wide enzyme galactosidase is responsible for lactose metabolism. This one encodes for the protein product that is also repressed, or which in the absence of lactose in the media and thus does not show fluorescence.

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