The temperature of the system is increased the number of collisions between with fluorophore in it's excited state and the quencher will increase.
Fluorophore is often affected by its environment. In the gas phase fluorescence lifetimes are the long because there is little interaction with bath molecules leading to non radiative deactivation. In condensed media the lifetime is a reporter of the local environment of the fluorophore. fluorescence is a very sensitive method for studying the local environment around the fluorophore. Two methods are very commonly employed to learn about the local environment on a molecular scale are quenching and Fluorescence Resonance Energy Transfer . Quenching is occurring by a collisional deactivation of the excited state, according to the kinetic molecular theory. The fluorescence signal will be smaller relative to the the intensity with the same concentration of quencher but at a lower temperature.
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