Because there are three recognition sites for restriction enzyme, cleaving a circular DNA yields three fragments of DNA.
What is DNA ?
- Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains that coil around one another to form a double helix. The polymer contains genetic instructions for all known organisms and viruses' development, functioning, growth, and reproduction. Nucleic acids include DNA and ribonucleic acid.
- Because they are made up of simpler monomeric units called nucleotides, the two DNA strands are known as polynucleotides. Each nucleotide is made up of one of four nitrogen-containing nucleobases, a deoxyribose sugar, and a phosphate group.
- Covalent bonds between the sugar of one nucleotide and the phosphate of the next nucleotide form a chain that results in an alternating sugar-phosphate backbone. To form double-stranded DNA, the nitrogenous bases of two separate polynucleotide strands are bound together with hydrogen bonds according to base pairing rules.
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