Practical process of determining the amino acid sequence of all or part of a protein or peptide is referred to as protein sequencing.
Protein sequencing is defined as the practical process of determining the amino acid sequence of all or part of a protein or peptide. This process can also help to identify the protein or characterize its post-translational modifications. Two main methods are used to deduce the amino acid sequence of proteins. These methods are Edman degradation and mass spectrometry-based amino acid sequencing. Mass spectrometry involves enzymatic or chemical degradation of the protein, and then a collection of peptides which are then fractionated by high-performance liquid chromatography. Edman degradation is used for characterizing a protein's N-terminus.
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