Perhaps this chromosome won't connect to your probe; It's possible that you overlooked adding the fluorophores molecule which binds to the fourth probe; Perhaps you forgot to add the fourth probe.
Chromosomes, etc that are organelles found in the nucleus of cells, are responsible for carrying lengthy sequences of DNA. DNA is the material that contains genes. It serves as the building block of the human body. Chromosome proteins contribute to the proper organization of DNA.
Since there are 23 pairs of these chromosomes in each human cell, the number of diploid human cells is 46, or 23 for the haploid human cell. 23 pairs total, 22 of these are recognized as autosomes. The 'X' and 'Y' chromosomes, or the 23rd pair, are responsible for determining sexuality.
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