A metabolic imbalance develops when E. coli grows in a medium with non-limiting quantities of glucose, and the main effect is the secretion of acetate. The viability and productivity of the strain are decreased by the generation of this harmful organic acid.
This issue can be resolved by using substrate feeding techniques to lower the glucose concentration or by creating mutant strains with reduced ability to import glucose.
In order to assess the impact of decreased glucose import capacity on the growth rate, biomass output, acetate, and synthesis of an experimental plasmid DNA vaccine, a collection of E. coli strains with dormant genes encoding proteins involved in glucose transport was created (PHN).
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