The CRISPR technology's Cas 9 enzyme is in charge of cutting the targeted section of the DNA exactly where the researcher wants to change the sequence.
When the targeted DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops it, turning off the gene. Instead of breaking the DNA, scientists can trigger gene expression by using customised versions of Cas9. Upon activation, the Cas9 protein randomly searches for the target DNA by attaching to regions that correspond to its protospacer adjacent motif (PAM) sequence. A two- or three-base sequence known as a PAM is found one nucleotide downstream of the guide RNA's complementary region.
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