The restriction endonuclease hind iii cut circular dna at three cut sites, which resulted in 3 restriction fragments.
HindIII is a type II site-specific deoxyribonuclease restriction enzyme identified from Haemophilus influenzae that hydrolyzes the cofactor Mg2+ to cleave the DNA palindromic sequence AAGCTT. restriction endonuclease with HindIII. Endonuclease In the presence of Mg2+, the type II restriction enzyme HindIII detects and breaks the palindromic sequence AAGCTT. The gene for HindIII endonuclease (R) is situated before the gene for its associated methyltransferase (M). HindII's cleavage produced what are termed as blunt-end fragments since it happened in the middle of a six-base pair recognition site.
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