Acetyl CoA serves as the cycle's substrate.
What is a substrate in a cycle?
The word "substrate" has a lot to do with context in chemistry. In general, it can refer to a chemical species that is being studied in a chemical reaction or a surface that is being used for microscopy or other chemical processes. In the first instance, a reagent is introduced to the substrate to cause a chemical reaction that will produce a product. In synthetic and organic chemistry, where the substrate is the chemical of interest that is being transformed, the word is used in a similar way. The substance that an enzyme reacts with is referred to as an enzyme substrate in biochemistry. In the latter meaning, it may refer to a surface on which further chemical processes are carried out or serve as an adjunct in a number of spectroscopic and microscopic procedures.
Acetyl CoA serves as the cycle's substrate. The Krebs cycle produces four reducing equivalents, consisting of three NADH and one FADH2, together with two CO2 and one high-energy phosphate bond as GTP.
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