The substrate concentration of the master mix is
a = 0.0016 mM
This is further explained below.
How do find the substrate concentration of the master mix?
Generally, Ethanol is acting as the substrate for this master mixture.
The following formula is used in order to determine the amount of ethanol that is included in the master mix:
CiVi= CfVf
Ci equals the starting concentration of ethanol, which is equal to 10 muM. 0.16 milliliters is equal to the volume of ethanol that was consumed initially.
Cf = final concentration, often known as the amount of ethanol present in the master mix = a Vf = final volume, which also refers to the final volume of the master mix = 1 milliliter
Introducing the values into the equation:
CiVi= CfVf
10 uM X 0.16 ml = a X 1 ml
a = (10 uM X 0.16 ml ) / 1ml
a = 1.6 uM
a = 1.6/ 1000 mM ... ( 1\muM = 1/1000 mM )
a = 0.0016 mM
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