Bacteria as a group are incredibly metabolically diverse, but individual species are often highly specialized to reduce competition in their natural environment. This results in these species being unculturable because.

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Bacterial species are unculturable because their growth may depend on necessary growth factors provided by other organisms in their natural environment.

Bacterial culture is a method to allow growth or proliferation of bacteria on a culture medium under controlled laboratory conditions. Two types of culture media include complete media and minimal media. Complete media provides all the nutrients and conditions required for bacterial growth while minimal media provides only the certain conditions.

Bacteria generally grow naturally in favourable conditions provided by the environment. In-vitro culturing of bacteria may not allow it to grow because of unfavourable conditions. Some reasons behind bacteria being unculturable include: the culture medium does not provide the essential nutrients, the culture medium is toxic to the bacterial growth, or some bacteria in the culture produces some substances that inhibits the growth of the target bacteria.

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