In a melting profile for DNA, the absorbance at 260 nm increases as a result of the disruption of the DNA structure. Denaturation is the fundamental physical basis for the absorbance increase
As a result of denaturation, many of the weak connections or bonds (such as hydrogen bonds) that give proteins their highly organized structure in their native (natural) state are broken. Most denatured proteins are insoluble and have a looser, more erratic structure.
Denaturation is the term used to describe how a protein unfolds or fragments, changing its typical three-dimensional structure. A protein's polypeptide chains may become disordered or unfurl due to chemical reaction, heat, or agitation, rendering the molecule normally non-functional. Egg whites are a perfect example of a denaturation that has been denaturized.
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