The large crystal violet-iodine complex is not able to penetrate this tightened peptidoglycan layer, and is thus trapped in the cell of Gram-positive bacteria could cause a gram-positive cell to lose its crystal violet-iodine complex.
At the end of the Gram staining procedure, Gram-positive cells are stained purple-blue. Gram-negative cells also take up crystal violet and iodine forms intracellular crystal violet-iodine complexes similar to Gram-positive cells.
Gram-positive cell organisms have high peptidoglycan content and Gram-negative organisms have high lipid content. First, all bacteria absorb crystal violet dye. However, the use of solvents dissolves the lipid layer of Gram-negative bacteria.
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