the fundamental physical basis for the absorbance increase is that the denatured DNA absorbs light more strongly than native DNA because the bases have become unstacked.
Deoxyribonucleic acid is a kind of DNA. All known animals and many viruses contain genetic instructions in the form of a polymer called DNA, which is made up of two polynucleotide chains that coil around one another to form a double helix.
Nucleic acids include DNA and ribonucleic acid (RNA). Nucleic acids are one of the four main categories of macromolecules that are necessary for all known forms of life, along with proteins, lipids, and complex carbohydrates (polysaccharides).
Because the two DNA strands are made up of simpler monomeric units termed nucleotides, they are referred to as polynucleotides.
Each nucleotide is made up of a phosphate group, a deoxyribose sugar, and one of the four nitrogen-containing nucleobases (cytosine [C], guanine [G], adenine [A], or thymine [T]).
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