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When a carboxylic acid is dissolved in isotopically labeled water, the label rapidly becomes incorporated into both oxygen atoms of the carboxylic acid.
What is Carboxylic acid ?
A functional group known as carboxyl is attached to the molecule carboxylic acid.
The substance R-COOH is a representation of carboxylic acid. They create a number of chemicals using the acidity of carboxylic acid.
Mechanism when carboxylic acid interacts with water:
- Step 1: The equilibrium between a hydrated carboxylic acid and its hydrated equivalent that develops when a water molecule is inserted over the C=O double bond.
- Step 2: The acid particles swiftly switch between these two states. Unlike the other three OH groups, which may all be expelled when moving to the left, the OH group acquired while going to the right comes from a water molecule in the solution. Unlabeled, mono-labeled, and doubly-labeled carboxylic acid molecules will consequently achieve equilibrium.
- Step 3: Water is designated as the solvent. As a result, it is more easier to reach labeled oxygen atoms than unlabeled ones. The aforementioned equilibriums are pushed to the right as a result, and the majority of acid molecules contain two specified oxygen atoms.
As a result, when a carboxylic acid is dissolved in isotopically labeled water, the label quickly binds to both of the acid's oxygen atoms.
To learn more about carboxylic acids, go at:
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