The transcriptional control of the cdkna protein can be studied using an RT-PCR assay to measure Cdkn1a mRNA levels.
The purpose of transcription factors (TFs), which bind to particular DNA sequences, is to activate (or, less frequently, inhibit) transcription of DNA. With up to 106-fold more affinity for their target sequences than for the rest of the DNA strand, TFs have distinct DNA-binding domains. The apparatus must be protected from enzyme deterioration by these transcriptional regulator proteins. Transcriptional regulatory proteins can detect DNA sequences and bind to them. They bind to regulatory sequences selectively and tightly. Such proteins' ability to bind to DNA can control transcription by blocking or enhancing transcription from a certain promoter.
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