The sample volume is constrained by the number of rotors that can be employed in the lab and by the methodology in use. A centrifuge tube should typically be filled to a minimum of 75%.
During centrifugation, which rapidly rotates the sample around a fixed axis to break it into its constituent parts, liquids are contained in centrifuge tubes.
The conical bottoms of most centrifuge tubes assist gather any solid or heavy components of the sample being centrifuged. The centrifugal pressure that is produced while centrifuge tubes are in operation must also be able to bear it. The maximum speed at which centrifuge tubes can be used safely may be specified in their specifications.
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