Question

What is the specific application of reverse transcriptase in the preparation of cdna?

Answer 1

The specific application involved in reverse transcriptase preparation of cDNA is a synthesis of DNA to form an RNA-DNA duplex.

What is reverse trancriptase activirt in cDNA?

• Recombinant RNA-dependent DNA polymerases known as reverse transcriptases are employed in molecular biology to catalyze the conversion of RNA into DNA during a first-strand cDNA synthesis reaction.
• A double helix molecule with an RNA-DNA hybrid is created by this process. When an RNA fragment is complexed with DNA, reverse transcriptases feature an RNase H domain with endoribonuclease activity that specifically breaks down RNA.
• The RNA fragment can only be transcribed once thanks to the degradation of RNA in an RNA-DNA complex, which minimizes transcription bias and ensures reaction fidelity.

Reverse transcriptases from either the avian myeloblastosis virus (AMV) or the Moloney murine leukaemia virus (MMLV) are used in the majority of commercial reverse transcription kits.

Compared to AMV, MMLV provides a number of benefits, but its reduced error rate and greater processivity rate stand out.

Hence, the correct answer is a synthesis of DNA to form an RNA-DNA duplex.

Learn more about RNA here,

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