The positive Biuret test indicates the presence of Peptide bond in proteins.
How to do Biuret test for proteins?
Follow these simple steps if you want to know practically:
Step 1: Take a 3 clean and dry test tubes.
Step 2: Add 1/2 ml of test solution to one test tube, albumin for another, and distilled water to third test tube.
Step 3: Add 3-4 drops of Biuret reagent to each test tube.
Step 4: Shake wee and let it be for 4-5 minutes.
Step 5: Observe the colour changes.
Peptide bonds are (C - N ) bonds present in proteins. The Biuret reagent colour is Pale blue and when you test for protein, if the solution contain protein, when you add the Biuret reagent it will turns to Purple. If there is no protein then the colour remains same.
The Biuret reagent is made up of Hydrated copper sulfate, sodium hydroxide, and Rochelle salt (sodium-potassium tartrate).
Peptide bond is also referred to as Amide bonds, it is formed between the Nitrogen atom of one amino acid and the Carbonyl carbon of second amino acid.
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