Respuesta :
The importance of the ionic strength of the buffer used in gel electrophoresis is (A) a heat-labile protein will become denatured if the ionic strength of the buffer is too high.
What is a buffer?
- A buffer solution is an aqueous solution composed of a weak acid and its conjugate base, or vice versa.
- When a modest amount of strong acid or base is introduced to it, the pH changes very little.
- According to the formula in Step 1, ionic strength is the squared sum of the concentrations and valences of all ions in the solution.
- Allow "C" to indicate the molar concentration of ions.
- There will be numerous concentrations to sum in mixed solutions.
- For all ions, the unit is moles per liter.
- The ionic strength of the buffer used in gel electrophoresis is important because a heat-labile protein will get denatured if the ionic strength of the buffer is too high.
Therefore, the importance of the ionic strength of the buffer used in gel electrophoresis is (A) a heat-labile protein will become denatured if the ionic strength of the buffer is too high.
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The complete question is given below:
What is the importance of the ionic strength of the buffer used in gel electrophoresis?
a. A heat-labile protein will become denatured if the ionic strength of the buffer is too high.
b. Increased ionic strength will lead to a backward electrophoretic pattern.
c. No migration will occur if a buffer's ionic strength is too high.
d. Staining of the protein fractions will not occur if the ionic strength in the buffer is too high.
