Methylation analysis and then hydrolysis are utilized to confirm the new oligosaccharide's glycosidic linkage information.
What is an oligosaccharide?
- When two or more monosaccharides bind together via O-glycosidic bonds, oligosaccharides are formed.
- When compared to other, more prevalent carbohydrates, including those in the disaccharide group, oligosaccharides, or carbohydrates with between 3 and 10 single sugar residues, are not as prevalent in the diet.
Examples of oligosaccharides: sucrose, lactose and maltose
What is Exhaustive methylation?
- Exhaustive methylation is the process of adding methyl groups to an alkyl ring until all methylations are complete.
- Extensive methylation transforms an amine into an alkylammonium salt prior to E2 elimination in the Hofmann elimination reaction sequence.
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