Question

11. In plant cells grown in the presence of a metabolic poison that specifically inhibits mitochondrial F1ATP synthase, one would expect:

Answer 1

In plant cells grown in the presence of a metabolic poison that specifically inhibits mitochondrial F1ATP synthase, one would expect: An increase in the pH difference across the inner mitochondrial membrane.

The electron transport chain can no longer pump electrons into the intermembrane space. The pH of the intermembrane space would increase, lowering the pH gradient and stopping ATP synthesis. The function of ATP synthase is to form ATP from ADP and inorganic phosphate (Pi) in the F1 sector. This is possible due to the energy derived from the gradient of protons that cross the inner mitochondrial membrane from the intermembrane space into the matrix via the Fo part of the enzyme.

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