When RNA polymerase synthesizes a new mRNA, interactions between the RNA polymerase, the DNA, and the RNA hold the complex together. When a hairpin loop forms in the nascent mRNA:

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18-07-2022
Biology

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When RNA polymerase synthesizes a new mRNA, interactions between the RNA polymerase, the DNA, and the RNA hold the complex together. When a hairpin loop forms in the nascent mRNA:

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shafaquehasnainvaidi shafaquehasnainvaidi · Answer 1 · 2022-07-26T12:26:22+02:00

When a hairpin loop forms in the nascent mRNA: The hairpin will destabilize the interaction and possibly lead to transcriptional termination.

Transcription in prokaryotes like E. coli is terminated either by a rho-dependent process or a rho-independent process. Intrinsic termination is controlled by the specific sequences of RNA .

When the termination process starts, the transcribed mRNA forms a stable secondary structural hairpin loop, also known as a stem-loop. Several uracil nucleotides follow this RNA hairpin. The uracil and adenine connections are exceedingly weak. NusA, a protein attached to RNA polymerase, attaches to the stem-loop structure so firmly that it momentarily stalls the polymerase.

The polymerase is pausing at the same time that the poly-uracil sequence is being transcribed. The RNA-DNA duplex can unwind and separate from the RNA polymerase because the weak adenine-uracil interactions reduce the energy of destabilization for the RNA-DNA duplex. Overall, transcription is terminated by the modified RNA structure.

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