Question

We used the enzyme, taq polymerase, which is derived from the thermus aquaticus bacterium, in our pcr procedure. Why is this enzyme important for efficiency in pcr?.

Answer 1

Taq polymerase enzyme important for efficiency in PCR because it is thermostable polymerases that remain active at high temperatures (90 -95  degree Celsius) a beyond the denaturation temperature of DNA.

PCR :

Polymerase chain reaction (PCR) is a laboratory technique for rapidly amplifying millions to billions of copies of a specific segment of DNA, which can then be used for further research.

Taq polymerase is a DNA polymerase derived from bacteria called Thermus aquaticus, which are naturally thermophilic and is a thermostable polymerases that remain active at temperatures higher than the temperature at which DNA is denatured. This allows PCR to be used on DNA samples without the need to add new DNA polymerases after each cycle.

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