Question

Some analytes must be derivatized to increase their column retention or detectability. Derivatization means Group of answer choices altering the chemical structure of the analyte to increase detection and specificity. adding fluorescent labels or combining the analyte with chiral reagents or other chemicals to increase detectability. removing dissolved gases in the solvent to produce a clear chromatogram. using multiple detectors to assist in identification.

Answer 1

Derivatization means adding fluorescent labels or combining the analyte with chiral reagents or other chemicals to increase detectability.

Some analytes must be derivatized to increase their column retention or detectability.

Retention time can be referred to as the amount of time a solute spends in the stationary and mobile phases of a column.

Detectability is the ability of an analyte to get detected in the mobile phase of chromatography.

The refractometer, fluorescence detector, and UV detector are the three most popular liquid chromatography detectors. These detectors increase the detectability.

For derivatization, the fluorescence detector are used.

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