We can confirm that If a plasmid is cut once we will be left with a singular linear strand of DNA, whilst if it is cut twice, we are left with two strands of DNA.
This has to do with the fact that plasmids contain circular DNA. Therefore, when we cut into a plasmid, we are breaking the circular DNA into a single strand. Slicing it again after will result of course in two strands of DNA.
Therefore, we can confirm that If a plasmid is cut once we will be left with a singular linear strand of DNA, whilst if it is cut twice, we are left with two strands of DNA.
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